@prefix ex: <https://sparql.uniprot.org/.well-known/sparql-examples/> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix schema: <https://schema.org/> .
@prefix sh: <http://www.w3.org/ns/shacl#> .

ex:74_enzymes_with_at_least_two_transmembrane_domains a sh:SPARQLExecutable,
        sh:SPARQLSelectExecutable ;
    rdfs:comment "Find Hydrolases (enzymes that catalyse the hydrolysis of various bonds) that have at least two transmembrane domains"@en ;
    sh:prefixes _:sparql_examples_prefixes ;
    sh:select """PREFIX rdfs: <http://www.w3.org/2000/01/rdf-schema#>
PREFIX up: <http://purl.uniprot.org/core/>

SELECT
  ?protein
WHERE {
 ?protein up:enzyme|up:annotation/up:catalyticActivity/up:enzymeClass ?enzymeClass ;
                   up:annotation ?transMembraneAnnotation .
 ?enzymeClass rdfs:subClassOf <http://purl.uniprot.org/enzyme/3.-.-.-> .
 ?transMembraneAnnotation a up:Transmembrane_Annotation .
} GROUP BY ?protein HAVING (COUNT(DISTINCT ?transMembraneAnnotation) >= 2)""" ;
    schema:keywords "enzyme",
        "transmembrane" , "count" , "enzyme classification" ;
    schema:target <https://sparql.uniprot.org/sparql/> .