@prefix ex:<https://purl.expasy.org/sparql-examples/neXtProt/> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix schema: <https://schema.org/> .
@prefix sh: <http://www.w3.org/ns/shacl#> .

ex:NXQ_00148 a sh:SPARQLExecutable,
        sh:SPARQLSelectExecutable ;
    rdfs:comment "Variants with normal \"ubiquitin-protein transferase activity\" and decreased or increased binding to UBE2D1 (example: BRCA1-p.Ile89Thr)"@en ;
    sh:prefixes _:sparql_examples_prefixes ;
    sh:select """PREFIX : <http://nextprot.org/rdf/>
PREFIX nextprot: <http://nextprot.org/rdf/entry/>
PREFIX nextprot_cv: <http://nextprot.org/rdf/terminology/>
PREFIX rdfs: <http://www.w3.org/2000/01/rdf-schema#>

SELECT distinct ?pfname ?impact_on_ubi_trsfrt_activity ?impact_on_binding_UBE2D1 WHERE {
 values (?ubi_trsfrt_activity ?no_impact ?UBE2D1 ?impact)
 { (nextprot_cv:GO_0004842 nextprot_cv:ME_0000003 nextprot:NX_P51668 nextprot_cv:ME_0000002) }
 ?pf a :Proteoform .
 ?pf rdfs:label ?pfname .
 ?pf :phenotypicVariation ?pv1 .
 ?pv1 :impactedObject / :term ?ubi_trsfrt_activity .
 ?pv1 :term ?no_impact .
 ?no_impact rdfs:label ?impact_on_ubi_trsfrt_activity .
 ?pf :phenotypicVariation ?pv2 .
 ?pv2 :impactedObject / :interactant ?UBE2D1 .
 ?pv2 :term ?sub_impact .
 ?sub_impact :childOf ?impact .
 ?sub_impact rdfs:label ?impact_on_binding_UBE2D1 .
}""" ;
    schema:keywords "PPI",
        "enzyme",
        "function",
        "phenotype",
        "tutorial",
        "variant" ;
    schema:target <https://sparql.nextprot.org/sparql> .