@prefix ex:<https://purl.expasy.org/sparql-examples/neXtProt/> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix schema: <https://schema.org/> .
@prefix sh: <http://www.w3.org/ns/shacl#> .
ex:NXQ_09676 a sh:SPARQLExecutable, sh:SPARQLSelectExecutable ;
  sh:prefixes _:sparql_examples_prefixes ;
  rdfs:comment '''Check there are no entries with expression profile annotations with evidence from EST with an expression level other than detected'''@en ;
  schema:keywords "QC", "expression", "ECO" ;
  schema:target <https://sparql.nextprot.org/sparql> ;
  sh:select '''PREFIX : <http://nextprot.org/rdf/>
PREFIX cv: <http://nextprot.org/rdf/terminology/>

select distinct ?entry where {
  ?entry :isoform ?iso.
  ?iso :expressionProfile /:evidence ?ev.
  ?ev :evidenceCode cv:ECO_0000009. # EST
  ?ev :expressionLevel ?level
  filter ( ?level not in (:Positive))
}''' .